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Introduction to Some metabolism analysis methods

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This article is intended to provide several analysis methods of a few important substances that they are well known. The first is bile acids analysis.


  Bile acid is an important component of bile and plays an important role in fat metabolism. Bile acid mainly exists in the intestinal and liver circulation system and acts as a protective role during recycling. Only a small amount of the bile acid enters the peripheral circulation.


  Bile acid analysis is of great importance to the research and development of clinical chemistry and bile acid drugs. As early as the ancient Chinese " Shennong's Herbal Classic " recorded the efficacy of carp gall. In addition, many important prescriptions, such as Bezoar powder and liushen pill, contain animal bile acids. Studies on bile acids in western countries began in the early 19th century, and quantitative studies began in the 1970s.


  The second is Catecholamines acids also known as pyrocatechol. Catecholamine is a neurotransmitter including catechins and amines. Catechins and amines are bound by an enzymatic process of l-tyrosine with sympathetic nerve, adrenal medulla and chromaffin cells. In general, catecholamine refers to norepinephrine (NA), adrenaline (Adr) and dopamine (DA). All three catecholamines are converted from tyrosine as precursors.


  As a neurotransmitter and hormone, catecholamine is closely related to people's health and disease. It is not only directly involved in behavioral activities or the regulation of neural functions such as blood pressure, heart rate, breathing and sleep, but also associated with some functional diseases such as schizophrenia and depression. Catecholamines Analysis began in the 1940s.With the deepening of the research on catecholamine, the analytical methods have been developing and improving all the time. At present, the research methods of molecular electrochemistry have become the leading one


  The third one is eicosanoids. Eicosanoids is a kind of bioactive unsaturated fatty acids, it is an important inflammatory factor, widely exists in body fluids and tissues, regulating many physiological and pathological processes. There are many kinds of eicosanoids in vivo, the content is low, and there are a lot of isomers. Therefore, the separation and analysis of eicosanoids in vivo are facing great challenges.


  As for eicosanoids analysis, Although there have been a lot of research on analytical methods, but still many problems waiting to solve. Neither extraction method has formed a standard extraction scheme for different biological samples. Therefore, the establishment of standardized extraction methods for different biological samples will greatly facilitate the eicosanoids analysis. The most powerful tool for analysis is the combination of chromatography-mass spectrometry. The characteristics of high sensitivity, high selectivity, high flux and high speed provide a good technical platform for the establishment of fast analytical methods. With the continuous development of various technologies, analytical methods will be continuously optimized and play a greater role in the research of biomarkers discovery, pathological mechanism and drug action mechanism.


  The last one is vitamins. There are two parts of vitamins analysis: water soluble vitamins analysis and fat soluble vitamins analysis.


  Vitamin is one of the important elements of human growth and development. At present, many foods are added with water-soluble vitamins, but there is a serious question whether they meet the requirements. Therefore, the development of research on it has certainly guiding significance for people's scientific intake and scientific control of the additive amount in food.


  Fat soluble vitamins are insoluble in water but soluble in fats and some organic solvents, including vitamin A, vitamin D, vitamin E and vitamin K. Fat soluble vitamins can be stored in large amounts in the body, mainly in the liver, so excessive intake will cause poisoning. Fat-soluble vitamins are becoming increasingly popular in clinical field. Thus, it is necessary to give reasonable attention to the analysis of the content and function of fat-soluble vitamins so that ensure the safety of use, maintain physical health.

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